Anti-infectives (Track)





ANTICANCER SCREENING OF CYANOBACTERIAL METABOLITES

Maria Estela Silva-Stenico, Ana Lúcia Tasca Gois Ruiz, João Ernesto de Carvalho, Elaine Crespim, Luiz Alberto Beraldo de Moraes, Eduardo J. Crevelin, Marli Fátima Fiore

Universidade de São Paulo, CENA, Lab. Ecologia Molecular de Cianobactérias, C.P. 96, 13400-970 Piracicaba-SP, Brazil

Abstract:

Cyanobacteria produce numerous and structurally diverse secondary metabolites, that may prove useful for the development of novel drugs, which include bioactive compounds with cytotoxic, antifungal, antiviral, anticancer, antimitotic, antimicrobial, antitumor, specific enzyme inhibition and immunosuppressive activities. In this study ten Brazilian cyanobacterial strains (Nostoc sp. CENA67, CENA68, CENA69, Microcystis aeruginosa NPCD-1, NPLJ-4, SPC777, M. panniformis SPC702, Sphaerocavum brasiliense SPC484, Cylindrospermopsis raciborskii 339-T3 and Brasilonema terrestre CENA116) were screened for their anticancer activity. The intracellular extracts were evaluated for in vitro anticancer activity against a panel of seven human cancer cell lines (U251 glioma, central nervous system; NCI-ADR/RES, ovary, phenotype of the multidrug resistance; 786-0, kidney; NCI-H460, lung non-small cell type; PC-3, prostate; HT29, colon; K562, leukemia) and one non-tumoral cell line (3T3, murine embryonal fibroblast). The dichloromethane extract of B. terrestre CENA116 and the ethanolic extract of Nostoc sp. CENA69 showed weak activity against a panel of seven human cancer cell lines, while the dichloromethane extract of Nostoc sp. CENA69 showed moderate activity. Mass spectrometry analysis revealed the presence of protease inhibitors in these extracts. The results showed antiproliferative potential of cyanobacterial intracellular extracts.